Detecting endotoxin activity in bovine serum using an automated testing system |
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| Authors: | Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Kenji TSUKANO Masakazu TSUCHIYA Jeffrey LAKRITZ |
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| Affiliation: | 1)School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimati, Bunnkyoudai, Ebetsu, Hokkaido 069–8501, Japan;2)College of Veterinary Medical Science, Ohio State University, 601 Vernon L. Tharp St., Columbus, OH 43210–1089, U.S.A.;3)Endotoxin and Microbial Detection, Charles River, 1023 Wappoo Road, Suite 43B, Charleston, SC 29407, U.S.A. |
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| Abstract: | The aim of the present study was to compare the ability of the commercially available portable test system (PTSTM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTSTM was not significantly different from that of the traditional LAL-based assays. The results using PTSTM correlated with those using KT (r2=0.963, P<0.001) or KC assays (r2=0.982, P<0.001). Based on these findings, the PTSTM could be applied as a simplified system to assess endotoxin activity in bovine serum. |
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| Keywords: | cattle endotoxin limulus amebocyte lysate portable test system serum |
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