A globotetraosylceramide (Gb4) receptor-based ELISA for quantitative detection of Shiga toxin 2e |
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| Authors: | Katsuhiro TOGASHI Shiho SASAKI Wataru SATO |
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| Affiliation: | 1)Yamagata Integrated Agricultural Research Center Swine Experiment Station, 1 Hamanaka, Yamagata 998–0112, Japan;2)Yamagata Shonai Livestock Hygiene Service Center, 139 Yokoyama, Yamagata 997–1301, Japan;3)Yamagata Mogami Livestock Hygiene Service Center, 2034 Kanazawa, Yamagata 996–0002, Japan |
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| Abstract: | Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb4) receptor by ELISA (Gb4-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb4-ELISA increased linearly with Stx2eB concentration in the range of 20–2,500 ng/ml. The Gb4-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease. |
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| Keywords: | ELISA globotetraosylceramide porcine edema disease Shiga toxin 2e |
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