| Abstract: | Abstract As a consequence of the BSE crisis in Europe, the components of meal produced from fish or warm-blooded animals are under stricter control. PCR-based techniques for species identification of fish meal have been developed and applied to meals produced from a single species each, including herring, capelin, anchovy, horse mackerel and blue whiting. DNA was extracted by means of the cationic detergent cetyltrimethylammonium bromide and a region of the mitochondrial cytochrome b gene was amplified using universal primers. The amplicon was further characterized by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) analysis. A number of fish meals could be differentiated due to their species-specific DNA patterns. |
