小麦1BL/1RS易位系1RS分子标记位点稳定性分析

为检测小麦1BL／1RS易位系1RS位点的稳定性，利用1RS上11个标记位点的PCR特异引物对21个小麦1BL／1RS易位系进行了分子检测。结果表明，21个1BL／1RS易位系中，66．7％的品种1RS的11个标记位点较为稳定，扩增出标记位点特异性带，但有33．3％的品种部分标记位点出现特异性带的丢失或添加，具有不同程度的位点变异，位点变异率最高达45．5％。对于1RS上的11种PCR特异引物，引物NOR-R1和APR1．3可稳定扩增出黑麦特异带，是在小麦遗传背景中鉴别黑麦1RS较为可靠的分子标记。

Analysis on the Stability of 1RS in the Wheat-rye 1BL/1RS Translocation Lines

In order to identify the stability of 1RS in these wheat-rye 1BL/1RS translocation lines, 11 1RS specific primer sets were used to detect the 1RS in 21 wheat-rye 1BL/1RS translocation lines. The results indicated that 66.7% of the 21 wheat-rye 1BL/1RS translocation lines had a stable 1RS and amplified the specific band. However, 33.3% of the lines had some variations, there were some deletions and additions of the specific band in 1RS. The highest variation frequency is 45.5%. In addition,among the 11 1RS specific primers used in the experiment, only the primer sets NOR-R1 and APR 1.3 amplified the special band in all samples, which provided a useful means for molecular tagging of rye chromosomes 1RS in wheat genetic background.