| 双峰驼线粒体DNA地高辛标记探针的制备及其应用 |
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| 引用本文： | 权洁霞,韩建林,门正明,张亚平,张国琪,鲁守玮.双峰驼线粒体DNA地高辛标记探针的制备及其应用[J].甘肃农业大学学报,1997(4). |
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| 作者姓名： | 权洁霞 韩建林 门正明 张亚平 张国琪 鲁守玮 |
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| 作者单位： | 中国科学院昆明动物研究所细胞与分子进化开放研究实验室，甘肃农业大学动物生产工程系，甘肃省武威濒危野生动物繁育中心 |
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| 基金项目： | 中国科学院细胞学分子进化开放研究实验室开放基金 |
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| 摘 要： | 利用地高辛标记技术，对从双峰驼肝脏和肾脏提纯的线粒体ＤＮＡ进行了标记，制成探针，并用它检测了包含于白细胞总ＤＮＡ中的微量线粒体ＤＮＡ。结果表明这一技术完全可以成功地用于微量线粒体ＤＮＡＲＦＬＰ的分析。
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| 关 键 词： | mtDNA，地高辛标记，探针，双峰驼 |
Preparation of mtDNA Probe Labeled with Digoxigenin and Its Application to mtDNA RFLP of Two humped Camel |
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| Quan Jiexia , Han Jianlin , Men Zhengming , Zhang Yaping Zhang Guoqi Lu Shouwei.Preparation of mtDNA Probe Labeled with Digoxigenin and Its Application to mtDNA RFLP of Two humped Camel[J].Journal of Gansu Agricultural University,1997(4). |
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| Authors: | Quan Jiexia Han Jianlin Men Zhengming Zhang Yaping Zhang Guoqi Lu Shouwei |
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| Institution: | Quan Jiexia 1,2 Han Jianlin 1,2 Men Zhengming 1,2 Zhang Yaping 1 Zhang Guoqi 3 Lu Shouwei 3 |
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| Abstract: | Through labeling mtDNAs being extracted from the tissues of liver or kidney of two humped camels by digoxigenin, the mtDNA probes were produced successfully. RFLPs of mini trace mtDNA contained in the total genomic DNA of white cells were detected. It was showed that this procedure was simple and effective in investigating the mtDNA RFLPs of camels or other mammals . |
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| Keywords: | mtDNA digoxigenin probe two humped camel |
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