| 银川市番茄白粉病病原菌的鉴定 |
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| 引用本文： | 周鹏泽,王晓敏,孔维康,王林,白圣懿,李小红,郭猛,程国新. 银川市番茄白粉病病原菌的鉴定[J]. 湖南农业大学学报(自然科学版), 2022, 48(2): 196-200. DOI: 10.13331/j.cnki.jhau.2022.02.011 |
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| 作者姓名： | 周鹏泽 王晓敏 孔维康 王林 白圣懿 李小红 郭猛 程国新 |
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| 作者单位： | 宁夏大学农学院,宁夏 银川 750021,宁夏大学农学院,宁夏 银川 750021;宁夏现代设施园艺工程技术研究中心,宁夏 银川 750021;宁夏优势特色作物现代分子育种重点实验室,宁夏 银川 750021;宁夏设施园艺(宁夏大学)技术创新中心,宁夏 银川 750021 |
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| 基金项目： | 国家自然科学基金委地区项目(31860561)；宁夏回族自治区科学技术厅重点研发项目(2019BBF02022)；2016年度教育部春晖计划项目(教外司[2016] 2083号) |
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| 摘 要： | 对宁夏银川市收集的番茄白粉病病叶上的病原菌进行分离、纯化，分别进行形态学和分子生物学鉴定。形态学鉴定结果表明：银川市番茄白粉病病原菌分生孢子单生，呈卵圆形或腰鼓状；其顶端位置萌发出芽管，芽管末端生有乳突状附着胞，附着胞亦着生于菌丝上；分生孢子梗直立不分支；未发现闭囊壳。对病原菌的内部转录间隔区序列进行比对，其与新番茄粉孢菌(Oidiumneolycopersici)的一致性达99%以上。形态学鉴定结合分子生物学鉴定结果表明，引起银川市番茄白粉病的病原菌为新番茄粉孢菌。
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| 关 键 词： | 番茄 白粉病菌 形态学鉴定 内部转录间隔区鉴定 银川市 |
Identification of pathogen causing tomato powdery mildew in Yinchuan |
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| ZHOU Pengze,WANG Xiaomin,KONG Weikang,WANG Lin,BAI Shengyi,LI Xiaohong,GUO Meng,CHENG Guoxin. Identification of pathogen causing tomato powdery mildew in Yinchuan[J]. Journal of Hunan Agricultural University, 2022, 48(2): 196-200. DOI: 10.13331/j.cnki.jhau.2022.02.011 |
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| Authors: | ZHOU Pengze WANG Xiaomin KONG Weikang WANG Lin BAI Shengyi LI Xiaohong GUO Meng CHENG Guoxin |
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| Abstract: | Pathogen of tomato powdery mildew in Yinchuan of Ningxia was isolated and purified, then characterized by morphology and molecular biology methods. Morphology of the pathogen showed that its conidia were solitary, oval or drum shaped; germ tubes germinated at the top of conidia, mastoid appressoriums formed at the end of germ tubes which also occurred on hyphae; conidiophores were erect and unbranched; no closed capsule was found. Internal transcribed spacer sequence alignment showed the pathogen have more than 99% consistency with the Oidium neolycopersici. Morphological and molecular biological identification showed that the pathogen causing tomato powdery mildew in Yinchuan was Oidium neolycopersici. |
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| Keywords: | tomato powdery mildew morphology identification internal transcribed spacer (ITS) identification Yinchuan |
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