Effect of 6-BA and 2, 4-D on Soybean Transformation |
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| Authors: | LI Haiyan LIU Miao SONG Xiaohui HAN Yingpeng WU Xiaoxia ZHANG Dayong LI Wenbin |
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| Affiliation: | Soybean Research Institute,Northeast Agricultural University,Harbin 150030,China |
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| Abstract: | Regeneration from cotyledonary nodes and embryonic tips of soybean"Peking"was studied.The disinfectant ways ofthe mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2,4-D were studied in the culture medium.The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride.The best concentration of 6-BA was 1.0 mg·L-1 and the best concentration of 2.4-D was 2.0 mg·L-1 in the germinating medium.The number of buds of each explant was 3.56 and 2.98,respectively.The best concentrations of 6-BA and 2,4-D were 3.0mg·L-1 and 3.5mg·L-1 in regeneration of embryonic tips.The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78,respectively. |
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| Keywords: | soybean(Glycine max L.) cotyledonary node embryonic tip hormone regeneration |
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