| 普通小麦(T.aestivum L.)Waxy蛋白种质资源研究 |
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| 引用本文： | 余春梅,陈佩度.普通小麦(T.aestivum L.)Waxy蛋白种质资源研究[J].南京农业大学学报,2003,26(3):1-6. |
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| 作者姓名： | 余春梅 陈佩度 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095 |
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| 摘 要： | 用SDS-PAGE方法对国内外293份小麦品种(系)Waxy蛋白进行了鉴定，筛选出缺Wx-A1的材料2份；缺Wx-B1的材料15份，其中14份材料为本研究首次发现；缺Wx-D1的材料2份；同时缺失Wx-A1和Wx-B1的材料2份；全缺的材料3份。利用分子标记对不同的Waxy蛋白缺失类型进行了鉴定，开发了一个Wx-A1位点的共显性STS标记。
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| 关 键 词： | 普通小麦 Waxy蛋白 种质资源 分子标记 SDS-PAGE 直链淀粉 基因缺失 |
| 文章编号： | 1000-2030(2003)03-0001-06 |
| 修稿时间： | 2003年4月9日 |
Study on common wheat (T. Aestivum L.) germplasm for Waxy protein |
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| YU Chun mei,CHEN Pei du.Study on common wheat (T. Aestivum L.) germplasm for Waxy protein[J].Journal of Nanjing Agricultural University,2003,26(3):1-6. |
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| Authors: | YU Chun mei CHEN Pei du |
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| Institution: | YU Chun mei,CHEN Pei du * |
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| Abstract: | The types of Waxy protein of 293 wheat varieties or lines introduced from various countries were identified by SDS PAGE method.Two varieties were identified to be the type with null in Wx A1 protein;fifteen with null in Wx B1,among them fourteen were discovered in the present research;two with null in Wx D1;two with null both Wx A1 and Wx B1 subunit and three null in all Wx protein.Different null types of Waxy protein were also identified by using PCR based molecular markers published by other researchers and a new co dominant STS(special tagged sequence)marker at Wx A1 locus was developed. |
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| Keywords: | common wheat Waxy protein SDS PAGE molecular marker |
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