| 均匀设计法优化北五味子愈伤组织诱导培养基 |
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| 引用本文： | 张佳,金东淳,金海林,刘权刚.均匀设计法优化北五味子愈伤组织诱导培养基[J].农业科学与技术,2011(1):141-143. |
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| 作者姓名： | 张佳 金东淳 金海林 刘权刚 |
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| 作者单位： | 延边大学农学院 |
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| 基金项目： | Supported by International Collaboration Research Program~~ |
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| 摘 要： | 目的]优化北五味子愈伤组织诱导培养基。方法]采用均匀设计法,以诱导率为主要考察目标,对北五味子愈伤组织生长的激素成分进行多因素多水平考察。结果]叶片的最佳培养基组合为MS＋3mg/L6-BA;叶柄的最佳培养基组合为MS＋3mg/L6-BA＋0mg/LNAA;茎段的最佳培养基组合为MS＋3mg/L6-BA＋0.6mg/LNAA。结论]用均匀设计法优化愈伤组织诱导培养基省时、方便,且明显高于均匀设计实验方案中的诱导率。
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| 关 键 词： | 北五味子 愈伤组织 均匀设计 诱导率 |
Optimization of Callus Induction Medium for Schisandga chinensis Baill via Uniform Design |
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| ZHANG Jia,JIN Dong-chun,JIN Hai-lin,LIU Quan-gang.Optimization of Callus Induction Medium for Schisandga chinensis Baill via Uniform Design[J].Agricultural Science ＆ Technology,2011(1):141-143. |
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| Authors: | ZHANG Jia JIN Dong-chun JIN Hai-lin LIU Quan-gang |
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| Institution: | Department of Agronomy,Agricultural College of Yanbian University,Yanji 133002 |
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| Abstract: | Objective]This study was to optimize callus induction medium for Schisandga chinensis Baill.Method]Using callus induction rate as an indicator,uniform design was employed to optimize hormone combination at poly-factors and poly-levels for callus induction from Schisandga chinensis Baill.Results]Optimal hormone combinations depended on different explants:optimum medium for both tender leaf and petiole was MS +3 mg/L 6-BA,for stem segment was MS +3 mg/L 6-BA +0.6 mg/L NAA.Conclusion]Uniform design is a time-saving and convenient method for the optimum medium for callus and yields a higher callus induction rate. |
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| Keywords: | Schisandga chinensis Baill Callus tissue Uniform design Callus induction rate |
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