| 反相高效液相色谱法测定三氮脒有关物质的方法学研究 |
| |
| 引用本文： | 金录胜,陈莎莎,李树纲,张宏,于清伟,张蕾. 反相高效液相色谱法测定三氮脒有关物质的方法学研究[J]. 中兽医医药杂志, 2010, 29(6): 41-43 |
| |
| 作者姓名： | 金录胜 陈莎莎 李树纲 张宏 于清伟 张蕾 |
| |
| 作者单位： | [1]甘肃省兽药饲料监察所,甘肃兰州730030 [2]中国兽医药品监察所,甘肃兰州730030 |
| |
| 基金项目： | 中国兽药典委员会2010年版《中国兽药典》科研项目 |
| |
| 摘 要： | 目的:采用RP-HPLC法测定三氮脒的有关物质。方法:使用ZORBAXExtend-C_(18)柱(4.6mm×250mm,5μm),流动相为0.025mol/L磷酸溶液(用三乙胺调节pH值至3.0)-乙腈(91:9),检测波长为254nm,流速为1.0mL/min。结果:三氮脒与各有关物质在上述色谱条件下能有效地分离。结论:该方法结果准确、操作简单、灵敏度高、专属性强,适用于三氮脒的有关物质测定。
|
| 关 键 词： | 三氮脒 有关物质 反相高效液相色谱法 |
Determination on related substances in diminazene aceturate by RP-HPLC |
| |
| JIN Lu-sheng,CHEN Sha-sha,LI Shu-gang,ZHANG Hong,YU Qing-wei,ZHANG Lei. Determination on related substances in diminazene aceturate by RP-HPLC[J]. Journal of Traditional Chinese Veterinary Medicine, 2010, 29(6): 41-43 |
| |
| Authors: | JIN Lu-sheng CHEN Sha-sha LI Shu-gang ZHANG Hong YU Qing-wei ZHANG Lei |
| |
| Affiliation: | (Gansu Provincial Institute of Veterinary Drug and Feed Control. Lanzhou Gansu 730030, China) |
| |
| Abstract: | Objective： The reversed phase high performance liquid chromatography method was established to determine the related substances in diminazene aceturate. Methods： The column was ZORBAX Extend-C18 （4.6mm×250mm,5μm）, the mobile phase was phosphoric acid solution （adjusted pH value to 3.0 by triethylamine）-acetonitrile （91：9） of 0.025 mol/L, the detection wave length was 254 nm, the flow rate was 1.0 mL/min. Results： Diminazene aceturate and all of the related substances could be separated efficiently with this condition. Conclusion： The method was accurate, simple, rapid and exclusive. It could be used for the determination of related substances in diminazene aceturate. |
| |
| Keywords: | diminazene aceturate related substances RP-HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
