Growth of facultative anaerobic population of human intestinal bacteria with the carbamate pesticide aminocarb |
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| Institution: | 1. Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Science, University Mohamed Premier, Oujda 60000, Morocco;2. Laboratory of Plant Biology and Microorganism, Faculty of Science, University Mohamed Premier, Oujda 60000, Morocco;3. Laboratory for the Improvement of Agricultural Production, Biotechnology and the Environment (LAPABE), Faculty of Science, Mohammed Premier University, Oujda, Morocco;1. Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Koencho 165, Kitami 090-8507, Japan;2. Graduate School of Engineering, Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan;1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China;2. School of Grain Science and Technology, Jiangsu University of Science and Technology, 2 Mengxi Road, Zhenjiang, 212003, China;3. Nanjing Dongshan Foreign Language School, 99 Shanggao Road, Jiangning District, Nanjing, 211103, China;1. College of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, China;2. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China;3. College of Food and Biological Engineering, Jiangsu University, Zhenjiang, China |
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| Abstract: | The effect of different concentrations of the carbamate pesticide, aminocarb (Matacil), on the growth and selection of facultative anaerobic bacteria and degradation of the pesticide by human endoflora of the intestinal tract was examined in vitro. Microorganisms were cultured under aerobic or anaerobic conditions, in nutrient broth and mineral media. The intestinal population was more sensitive to 10–1000 μg/ml aminocarb under anaerobic conditions than in aerobic culture; however, spontaneous degradation of aminocarb in media markedly affected the degree of bacterial growth inhibition in prolonged cultures. In addition, the type of culture medium appeared to influence the degree of aminocarb-induced bacterial growth inhibition. A dose of aminocarb inducing 50% growth inhibition was established for different culture conditions: for mineral medium, aminocarb inhibited bacterial growth by 50% at 600 μg/ml under anaerobic and aerobic conditions, whereas less than 50% inhibition was observed even at 1000 μg/ml aminocarb when bacteria were grown in nutrient broth. A selection of bacterial strains occurred in the presence of increasing aminocarb concentrations, which was determined quantitatively and qualitatively by the identification of codominants. A shift in several Escherichia coli biotypes was also observed in cultures with aminocarb, in comparison to control cultures. Bacterial degradation of aminocarb, under anaerobic and aerobic conditions, was determined in a mixed population of the intestinal microflora by high-performance liquid chromatography analysis of culture media. Data showed that aminocarb can be quickly degraded by human intestinal bacteria at relatively high pesticide concentrations. Moreover, other HPLC data suggest rapid spontaneous degradation of aminocarb in neutral and slightly alkaline pH conditions characteristic of the human intestinal tract, which can effectively eliminate the pesticide. Therefore, aminocarb, at the concentrations used, does not seriously affect the bacterial microflora of the human gut. |
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