气相色谱法同时检测水果中氟吡菌酰胺、肟菌酯及其代谢物肟菌酸的残留量

采用气相色谱法建立了水果中氟吡菌酰胺、肟菌酯及其代谢物肟菌酸残留的分析方法。样品用乙腈匀浆提取，氯化钠和无水硫酸镁盐析后，经N-丙基乙二胺（PSA）分散固相萃取净化，气相色谱-电子捕获检测器（GC-ECD）检测。结果表明:在0.05~1 mg/kg添加水平下，氟吡菌酰胺、肟菌酯及其代谢物肟菌酸的平均回收率为79%~120%，相对标准偏差（RSD）为0.7%~16%。氟吡菌酰胺、肟菌酯及其代谢物肟菌酸在不同水果样品中的检出限（LOD）分别为0.01、0.015和0.01 mg/kg，定量限（LOQ）均为0.05 mg/kg。该方法快速、简单和稳定，可以满足水果中氟吡菌酰胺、肟菌酯及其代谢物肟菌酸残留量的检测要求。

Simultaneous determination of fluopyram,trifloxystrobin and its metabolite trifloxystrobin acid in fruits by gas chromatography

A method was established for determining fluopyram,trifloxystrobin and its metabolite trifloxystrobin acid residued in fruits by gas chromatography(GC).Residues were extracted with acetonitrile,salted-out by sodium chloride and magnesium sulfate,cleaned up by primary secondary amine (PSA),and determined by GC equipped by ECD.The results showed that the method was validated using fruits samples spiked with pesticides at concentration levels ranging from 0.05 to 1 mg/kg.The average recovery is from 79% to 120%,with relation standard deviations between 0.7% to 16%.The limits of detection (LOD) were 0.01,0.015 and 0.01 mg/kg for fluuopyram,trifloxystrobin and trifloxystrobin acid in the different fruits samples.And the limit of quantification (LOQ) was 0.05 mg/kg. The method is rapid,easy and stable for determination of fluopyram,trifloxystrobin and trifloxystrobin acid in fruits.