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天达V901草菇菌丝生长条件的筛选与优化
引用本文:曲玲,任海霞,任鹏飞,万鲁长,单洪涛,李瑾,韩建东.天达V901草菇菌丝生长条件的筛选与优化[J].山东农业科学,2012,44(10).
作者姓名:曲玲  任海霞  任鹏飞  万鲁长  单洪涛  李瑾  韩建东
作者单位:山东省农业科学院农业资源与环境研究所,山东济南,250100
基金项目:山东省农业良种工程重大课题"食用菌新品种培育"
摘    要:采用单因素试验和正交试验相结合的方法,研究了天达V901草菇菌丝在不同营养条件和不同培养条件下的生长状况。结果表明,适宜草菇菌丝生长的碳源有葡萄糖、纤维素、可溶性淀粉、麦芽糖和蔗糖,但对草菇菌丝生长最有利的碳源是可溶性淀粉;适宜草菇菌丝生长的最佳氮源是蛋白胨;最佳碳氮比是10∶1;最佳可溶性淀粉浓度是5.0%;钾对草菇菌丝生长有明显促进作用。草菇菌丝生长的最适温度为35℃;最适pH值为7.5。

关 键 词:草菇  菌丝生长  生物学特性  生长条件优化

Screening and Optimization of Conditions for Mycelia Growth of Volvariella volvacea Tianda V901
Qu Ling , Ren HaiXia , Ren PengFei , Wan LuZhang , Shan HongTao , Li Jin , Han JianDong.Screening and Optimization of Conditions for Mycelia Growth of Volvariella volvacea Tianda V901[J].Shandong Agricultural Sciences,2012,44(10).
Authors:Qu Ling  Ren HaiXia  Ren PengFei  Wan LuZhang  Shan HongTao  Li Jin  Han JianDong
Abstract:The growth situation of Volvariella volvacea(Bull.ex Fr.) Sing mycelia under different nutrient and culture conditions was studied by single factor experiment and orthogonal experiment.The results showed that the suitable carbon sources for mycelia growth were glucose,cellulose,soluble starch,maltose and sucrose,but the optimal carbon source was soluble starch.The optimal nitrogen source for mycelia growth was peptone.The optimal C/N was 10∶ 1.The optimal concentration of soluble starch was 5.0%,and potassium obviously promoted the mycelia growth.The optimal temperature and pH value for mycelia growth were 35℃ and 7.5 respectively.
Keywords:Volvariella volvacea(Bull  ex Fr  ) Sing  Mycelia growth  Biological characteristics  Optimization of growth conditions
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