| 胸膜肺炎放线杆菌诊断抗原的制备及初步临床应用 |
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| 引用本文： | 陈凡,何启盖,陈焕春,吴斌,刘正飞,Ross Bowle,Pat Blackall.胸膜肺炎放线杆菌诊断抗原的制备及初步临床应用[J].华中农业大学学报,2003,22(5):478-482. |
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| 作者姓名： | 陈凡 何启盖 陈焕春 吴斌 刘正飞 Ross Bowle Pat Blackall |
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| 作者单位： | 1. 华中农业大学动物医学院,武汉,430070
2. Animal Health Institute, Yeerongpilly, Queesland, Australia 4105 |
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| 基金项目： | 湖北省重点科技攻关项目(2001AA20lB020)资助 |
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| 摘 要： | 利用14个血清型的胸膜肺炎放线杆菌的标准菌株制备诊断抗原，通过玻片凝集试验，琼脂扩散试验和对流免疫电泳，以标准抗血清作为参考，检验各种诊断抗原的灵敏性和特异性。分别用凝集抗原、超声波处理抗原和酚水抗原对来自湖北、湖南、江西、河南、安徽5省猪场的155份送检血清进行检测。结果表明可以对送检血清快速、准确地进行鉴别诊断和血清分型。
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| 关 键 词： | 胸膜肺炎 放线杆菌 诊断抗原 灵敏性 特异性 血清 猪 |
| 修稿时间： | 2003年4月21日 |
The Preparation of 14 Serotypes Standard Diagnostic Antigens of Actinobacillus pleuropneumoniae and Its Clinic Application |
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| Ross Bowle,Pat Blackall.The Preparation of 14 Serotypes Standard Diagnostic Antigens of Actinobacillus pleuropneumoniae and Its Clinic Application[J].Journal of Huazhong Agricultural University,2003,22(5):478-482. |
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| Authors: | Ross Bowle Pat Blackall |
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| Abstract: | To test the sensitivity and specificity of various antigens, agglutination test, agar diffusion test and counter immunoelectrophoresis test are conducted, using standard strains of 14 Actinoba-cillus pleuropneumoniae serotypes as diagnostic antigens and standard antisera as reference. 155 sera samples form Hubei, Hunan , Jiangxi , Henan , Anhui were assayed with agglutination antigens . cap-sular antigens . phenol extraction antigens. The results showed that detection and identification of sera samples with these three antigens can be carried out in a fast and accurate way. |
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| Keywords: | Actinobacillus pleuropneumoniae diagnostic antigen serotype |
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