龙眼子叶胚3-磷酸甘油醛脱氢酶基因的cDNA克隆及序列分析

从龙眼子叶胚中分离得到一个大量表达的表达序列标签(EST),该EST编码序列与金鱼草3-磷酸甘油醛脱氢酶(GAPDH)基因的同源性为84%,利用cDNA末端快速扩增(RACE)技术成功克隆了龙眼GAPDH基因全长序列.序列分析表明,龙眼GAPDH基因的cDNA全长为1395 bp,包括一个长1008 bp、编码336个氨基酸的开放阅读框(ORF),5′端非编码区(UTR)长71 bp,3′-UTR长316 bp.龙眼GAPDH基因编码的氨基酸序列与水稻、葡萄、小果野蕉、拟南芥、银杏等GAPDH基因的氨基酸序列同源性均高达85%以上,该基因在GenBank中的登录号为FJ694011.

Cloning and sequence analysis of the full-length cDNA of glyceral-dehyde-3-phosphate dehydrogenase gene from longan cotyledon embryo

On the basis of one cDNA-AFLP expressed sequence tag (EST) from longan (Dimocarpus longan Lour. ) cotyledon embryos which is 84% homologous to Antirrhinum majus, the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was cloned by rapid-amplification of cDNA ends (RACE). The gene consisted of 1395 bp encoding a protein of 336 amino acids, with 71 bp and 316 bp at 5' - UTR and 3' - UTR, respectively. The amino acid sequence comparison with the GAPDH gene of Oryza saliva, Vitis vinifera, Musa acuminata, Arabidopsis thaliana, and Ginkgo biloba showed that identity was all higher than 85%. The sequence was accepted and released by GenBank (accession number; FJ694011 ).