| 航天搭载雪里蕻小孢子培养获得再生植株 |
| |
| 引用本文： | 虞慧芳,钟新民,顾宏辉,李必元,张晓辉. 航天搭载雪里蕻小孢子培养获得再生植株[J]. 浙江农业学报, 2007, 19(5): 356-359 |
| |
| 作者姓名： | 虞慧芳 钟新民 顾宏辉 李必元 张晓辉 |
| |
| 作者单位： | 浙江省农业科学院,蔬菜研究所,浙江,杭州,310021 |
| |
| 摘 要： | 对经"实践八号"育种卫星搭载雪里蕻进行小孢子培养研究。结果表明:雪里蕻小孢子分离12 d后肉眼可见胚状体,28 d后获得子叶期胚状体;雪里蕻平均每花蕾胚状体产量为6.1个;在不含激素B5培养基中的一次萌发率为38%,在含有激素的B5-1培养基中为27%;在含有激素的B5-2培养基中的次生萌发率为62%;共获得165株雪里蕻再生植株。
|
| 关 键 词： | 航天育种 雪里蕻 小孢子培养 |
| 文章编号： | 1004-1524（2007）05-0356-04 |
| 收稿时间： | 2007-06-08 |
| 修稿时间： | 2007-06-08 |
Obtaining microspore-cultured plants in potherb mustard,Brassica juncea var.multiceps,by space-breeding satellite |
| |
| YU Hui-fang,ZHONG Xin-ming,GU Hong-hui,LI Bi-yuan,ZHANG Xiao-hui. Obtaining microspore-cultured plants in potherb mustard,Brassica juncea var.multiceps,by space-breeding satellite[J]. Acta Agriculturae Zhejiangensis, 2007, 19(5): 356-359 |
| |
| Authors: | YU Hui-fang ZHONG Xin-ming GU Hong-hui LI Bi-yuan ZHANG Xiao-hui |
| |
| Affiliation: | Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hartgzhou 310021, China |
| |
| Abstract: | A useful procedure was described for the microspore culture in potherb mustard(Brassica juncea var.multiceps) whose seeds had been carried back by China's seed-breeding satellite,Shijian-8.The results showed that during microspores culture,embryos were visibly observed within 12 days after isolation,and cotyledon-shaped embryos were obtained within 28 days.About 6.1 embryos per flower bud were obtained on average.The first germinated rate for embryos was 38 percent in B5 solid medium without hormone,and 27 percent in B5-1 solid medium with hormone,while the second germinated rate was 62 percent in B5-2 with hormone.A total of 165 regenerated plants were obtained. |
| |
| Keywords: | space breeding potherb mustard(Brassica juncea var.multiceps) microspore culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
