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Chromosome and plasmid-encoded N-acyl homoserine lactones produced by Agrobacterium vitis wildtype and mutants that differ in their interactions with grape and tobacco |
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| Authors: | Yaxin Li Matthew R. Gronquist Guixia Hao Michele R. Holden Anatol Eberhard Russell A. Scott Michael A. Savka Erno Szegedi Sandor Sule Thomas J. Burr |
| Affiliation: | aDepartment of Plant Pathology, NYSAES, Cornell University, Geneva NY 14456, USA;bDepartment of Chemistry, SUNY College at Cortland, Cortland NY 13045, USA;cDepartment of Microbiology, Cornell University, Ithaca, NY 14853, USA;dDepartment of Biological Sciences, Rochester Institute of Technology, 85 Lomb Memorial Dr., Rochester, NY 14623, USA;eResearch Institute for Viticulture and Enology, 6000 Kecskemet, POB 25, Hungary;fPlant Protection Institute of Hungarian Academy of Sciences, POB 102, 1525 Budapest, Hungary |
| Abstract: | Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8–16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids. |
| Keywords: | Autoinducer Agrobacterium tumefaciens Rhizobiaceae Quorum-sensing Hypersensitive response |
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