| 应用单克隆抗体ELISA阻断试验快速检测兔出血症病毒抗体的研究 |
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| 引用本文： | 孙智锋 杜念兴. 应用单克隆抗体ELISA阻断试验快速检测兔出血症病毒抗体的研究[J]. 上海畜牧兽医通讯, 1996, 0(1): 3-5 |
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| 作者姓名： | 孙智锋 杜念兴 |
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| 作者单位： | 上海市农业科学院畜牧兽医研究所，南京农业大学动物医学院，南京农业大学动物医学院 上海 200030，南京 210095，南京 210095 |
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| 摘 要： | 利用抗兔出血症病毒(RHDV)的特异性单克隆抗体(McAb)建立了ELISA阻断试验用以检测血清抗体,并与血凝抑制(HI)和琼脂扩散试验(ID)进行了比较。结果ELISA阻断试验敏感性超过HI,更远胜于ID,具有灵敏、特异、准确等优点,适于大批血清样品的快速检测。
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| 关 键 词： | 兔出血症病毒 单克隆抗体 单抗ELISA阻断试验 |
Detection of Antibodies to Rabbit Hemorrhagic Disease Virus by Double-sandwich-blocking ELISA Using Monoclonal Antibodies |
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| Sun Zhifeng. Detection of Antibodies to Rabbit Hemorrhagic Disease Virus by Double-sandwich-blocking ELISA Using Monoclonal Antibodies[J]. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 1996, 0(1): 3-5 |
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| Authors: | Sun Zhifeng |
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| Abstract: | A double-sandwich-blocking ELISA coated with mixture of RM-14 and RM-17 was developed for thedetection of antibodies directed against rabbit hemorrhagic disease virus (RHDV) in blood serum of rabbits. It wasfand to be a sensitiye,specific,rapid,easily performed and accurate test as compared with the hemagglutinationimbition (HI) and immunodiffusion (ID) tests.suitable for the detection of RHDV antibodies in large quantities ofserum samples. |
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| Keywords: | Rabbit hemorrhagic disease virus (RHDV) Monoclonal antibody Double-sandwich-blocking ELISA. |
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