花生Argonaute 基因家族全基因组鉴定与表达模式分析

AGO蛋白（Argonaute protein）是RNA诱导沉默复合体的关键组分，在植物生长发育中发挥重要作用。花 生基因组测序的完成为全基因组水平上分析AGO抗病基因提供了条件。利用AGO蛋白的保守域在花生基因组数 据库与NCBI中进行同源比对，鉴定得到花生AGO 基因家族所有成员。我们基于生物信息学对AGO蛋白家族的进 化关系、理化性质、染色体定位、基因结构、结构域、不同组织中和胁迫下的表达模式等进行分析。结果表明：试验 共鉴定得到51个花生AGO 基因，包括12个A.duranensis 基因，12个A.ipaensis 基因以及27个栽培种花生AGO 基因。 染色体定位分析结果显示这些基因不均匀地分布在花生染色体上，且A.duranensis 与A.ipaensis 基因组上有10对成 员存在较为明显的同源关系。表达模式分析表明AdAGO2、AiAGO4、AdAGO3、AiAGO7、AdAGO8、AiAGO8 基因在花生 22个组织中整体表达量偏高；而花生茎尖（Shoot Tip）与雄蕊（Stamens）中AGO 基因家族呈现较高表达量。本研究结 果为揭示AGO蛋白功能和发掘花生的抗逆育种靶向基因资源提供了一定的理论依据。

Genome-wide identification and expression pattern analysis of peanut argonaute gene family

AGO(Argonaute) protein is a core component of RNA induced silencing complex (RISC) and plays an important role in plant growth and development. The completion of peanut genome sequencing provides the con? dition for analyzing the AGO gene family on the whole genome. The conserved domain of AGO protein was used to obtain the members of the peanut AGO gene family which was based on the Peanut base database and NCBI. The phylogenetic trees, physicochemical properties, chromosomal distribution, genes structure，domain structure, and gene expression patterns in 22 tissues and under stresses were analyzed. The results showed that 51 members of the peanut AGO genes including 12 of the A.duranensis genomes, 12 of the A.ipaensis genomes and 27 of the peanut cul? tivars were identified,. Chromosomal location analysis showed that these members were unevenly distributed on the peanut chromosomes, and 10 pairs of AGO members from the A.duranensis and A.ipaensis genomes had a significant homology relationship. The expression pattern analysis shown that AdAGO2, AiAGO4, AdAGO3, AiAGO7, AdAGO8, and AiAGO8 had high-level expression in 22 tissues of peanut; and the AGO gene family shown higher expression in peanut shoot tip and stamens than other tissues. This study provided some theoretical evidence for further