虎杖苷对牛卵母细胞体外成熟的影响

为研究虎杖苷(PD)对牛卵母细胞体外成熟的影响,本实验在体外成熟液中添加不同浓度(0、0.5、1.0、2.0μmol/L)PD,对牛卵母细胞体外培养22~24 h,统计第一极体排出率,然后对各组卵母细胞进行体外受精并统计胚胎的发育情况;最后分析PD对牛卵母细胞体外成熟作用机制。结果表明:与对照组(0μmol/L)相比,PD各处理组的第一极体排出率显著提高,体外受精后的胚胎分裂率无差异,而1.0μmol/L PD处理组囊胚率较对照组显著提高,1.0、2.0μmol/L PD处理组囊胚期细胞数较对照组显著提高;进一步分析发现,与对照组相比,1.0μmol/L PD可显著降低细胞内活性氧水平,提高抗氧化基因GPX4、SOD1表达水平,显著提高细胞内总谷胱甘肽含量。综上,成熟液中添加1.0μmol/L PD可提高细胞内抗氧化基因的表达以及提高谷胱甘肽含量,降低细胞内的活性氧含量,从而有利于牛卵母细胞的成熟以及早期胚胎发育。

Effects of Polydatin on in vitro Maturation of Bovine Oocytes

This study was undertaken to investigate the effect of polydatin on maturation of bovine oocytes in vitro. In the experiment, bovine oocytes were cultured in IVM medium supplement with 0, 0.5, 1.0, 2.0 μmol/L polydatin for 22-24 h. The polar body excretion rate was calculated. Then the oocytes were fertilized in vitro,and the development of IVF embryos was further analyzed. Finally, the mechanism of PD on IVM of bovine oocytes was analyzed. Our results showed that the polar body rate was significantly increased in each treatment group of PD, the cleavage rate of embryos was not significant different, the blastocyst rate in the 1.0 μmol/LPD group was significantly higher than that of the control group. Meanwhile, the number of cells in the blastocyst cells were significantly increased in the 1.0, 2.0 μmol/LPD treatment group than that of control group. The above results indicated that 1.0 μmol/L PD is the optimal concentration to be added to the in vitro maturation solution. Further analysis found that, compared to the control group, 1.0 μmol/L PD group significantly reducing intracellular ROS levels, increasing the expression level of GPX4, SOD1, increase the intracellular total GSH level. In conclusion, these results indicated that adding with 1.0 μmol/L PD into IVM medium is good for oocyte maturation.