| 辐照对脱水高丽菜微生物ATP生物发光光谱的影响 |
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| 引用本文： | 冯敏,肖欢,朱佳廷,林家彬,唐玉新,杨萍,王德宁,李行通.辐照对脱水高丽菜微生物ATP生物发光光谱的影响[J].核农学报,2010,24(6):1198-1202. |
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| 作者姓名： | 冯敏 肖欢 朱佳廷 林家彬 唐玉新 杨萍 王德宁 李行通 |
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| 作者单位： | 江苏省农业科学院原子能农业利用研究所,江苏南京,210014 |
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| 基金项目： | 农业部公益性行业专项经费项目，江苏省自主创新资金项目 |
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| 摘 要： | 以脱水高丽菜为材料，研究辐照对微生物ATP生物发光光谱的影响。结果表明：ATP标准品生物发光光谱的发光区域在490~640nm的范围内,峰时为563nm；辐照后样品中微生物ATP生物发光光谱的发光区域未发生改变；不同剂量辐照处理后，样品中微生物ATP提取液生物发光光谱的峰时没有显著差异；辐照后样品中微生物ATP生物发光光谱峰值高于对照，说明辐照后微生物ATP提取液中ATP的浓度有所增加，而且这种影响在相当长的一段时间内依然存在。
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| 关 键 词： | 辐照 微生物 ATP生物发光 光谱 |
EFFECT OF IRRADIATION ON BIOLUMINESCENCE SPECTRUM OF MICROBIAL ATP |
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| FENG Min,XIAO Huan,ZHU Jia-ting,LIN Jia-bin,TANG Yu-xin,YANG Ping,WANG De-ning,LI Xing-tong.EFFECT OF IRRADIATION ON BIOLUMINESCENCE SPECTRUM OF MICROBIAL ATP[J].Acta Agriculturae Nucleatae Sinica,2010,24(6):1198-1202. |
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| Authors: | FENG Min XIAO Huan ZHU Jia-ting LIN Jia-bin TANG Yu-xin YANG Ping WANG De-ning LI Xing-tong |
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| Institution: | 1.Institute for Application of Atomic Energy, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014;
2. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, Jiangsu 225009 |
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| Abstract: | The effect of irradiation on bioluminescence spectrum of dehydrated cabbage microbial ATP was studied. The results showed that the spectral bandwidth of ATP standard was from 490 to 640 nm and the peak wavelength was at 563 nm. The spectral bandwidths of irradiated dehydrated cabbage microbial ATP and CK did not change. Peak wavelengths of dehydrated cabbage irradiated at different dosages were not significantly different from that of CK. The peaks of bioluminescence spectrum of irradiated samples were higher than that of CK, which may be because of the increasing concentration of ATP, and this effect would be kept for quite a long time after irradiation. |
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| Keywords: | irradiation microorganism ATP bioluminescence spectrum |
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