PTEN 基因在犬乳腺肿瘤组织中的表达及临床意义

有关酪氨酸磷酸酶基因（Phosphatase and tensin homolog deleted on chromosometen,PTEN）在乳腺肿瘤中的检测在人医早有报道。为了研究PTEN基因在犬乳腺肿瘤组织中的表达情况,笔者运用实时荧光PCR定量检测了38例不同的犬乳腺肿瘤组织（包括15例良性乳腺肿瘤和23例恶性乳腺肿瘤）、4例正常犬乳腺组织。结果发现：PTEN基因在犬恶性乳腺肿瘤组织中表达量明显低于其在良性乳腺肿瘤和正常乳腺组织中的表达量,两者差异极显著（P〈0.001）;PTEN在良性乳腺肿瘤组织中的表达与正常犬乳腺组织相比,差异不显著（P〉0.05）;发生了淋巴结转移的乳腺癌PTEN基因的表达量与未发生转移的乳腺癌组织的表达量间差异亦不显著（P〉0.05）,且PTEN的表达量与肿瘤组织的大小和发病动物年龄无关。结论：PTEN蛋白表达异常可能与乳腺肿瘤发生、发展相关,可考虑作为判断犬乳腺肿瘤生物学行为和预测的指标。

Expression of PTEN Gene and Its Clinical Value in Canine Mammary Tumors

Elevated levels of phosphatase and tensin homolog deleted on chromosometen(PTEN) have been reported in mammary gland tumors in humans.This study was designed to study the expression of PTEN mRNA in canine mammary tumor tissues.We investigated 38 canine mammary gland tumors(including 15 benign mammary tumors and 23 malignant mammary tumors),4 normal mammary gland by real-time RT-quantitative PCR.PTEN gene expression levels in canine malignant mammary tumors were much lower than that in benign mammary tumors and normal mammary tissues(P < 0.001);The expression of PTEN in benign mammary tumors vary insignificantly than that in normal mammary tissues(P>0.05);The expression of PTEN in canine mammary cancer without lymph node metastasis vary insignificantly contrast to mammary cancer with lymph node metastasis(P>0.05).The expression of PTEN was not correlated with tumor size and patient' age.In conclusion,the abnormal expression of PTEN gene protein plays an important role in the development of canine mammary tumors.It may be a predictor for biological behaviors and prognosis of breast cancer.