盐芥EsABI1基因克隆及其功能预测

目的]对盐芥EsABI1基因进行克隆及功能预测。方法]克隆盐芥EsABI1基因c DNA序列,对其蛋白保守结构域和系统进化进行分析。结果]EsABI1与At ABI1属于进化上的同一分支,有最近的亲缘关系,都含有包含PP2C蛋白磷酸酶催化活性位点在内的11个保守功能域。定量PCR检测发现,盐芥EsABI1沉默植株中EsABI1表达水平较野生型均有不同程度的降低。60μmol/L ABA分别处理EsABI1沉默植株0、3和6 h后,发现EsABI1基因表达水平受ABA诱导上调,而且相比于盐芥野生型,EsABI1沉默植株中EsABI1受ABA诱导上调表达更加明显。结论]EsABI1是ABA信号转导途径中的负调控因子,参与了盐芥对环境胁迫的响应。

Cloning of EsABI1 of Eutrema salsugineum and Its Function Prediction

Objective]To clone the EsABI1 of Eutrema salsugineum and predict the function.Method]The cDNA sequence of EsABI1 of E.sal-sugineum was cloned to study the protein conserved domain and phyletic evolution.Result]EsABI1 and AtABI1 had highest similarity and be-longed to one branch in evolution,which contained 11 conserved domains,including PP2C protein phosphatase catalytic active site.Quantitative PCR detection showed that the expression level of EsABI1 in EsABI1 silent plants was lower than that in the wild type.The expression of EsABI1 had been up-regulated under 60μmol/L ABA for 0,3,6 hours in E.salsugineum,and the higher up-regulated expression of EsABI1 compared with wide type.Conclusion] EsABI1 acted as a negative regulator in ABA signal transduction pathway of E.salsugineum,which participated in the re-sponse of E.salsugineum to environmental stress.