费乌瑞它马铃薯脱毒技术研究

[目的]解决费乌瑞它马铃薯种薯退化问题。[方法]利用植物茎尖脱毒培养技术对费乌瑞它马铃薯进行脱毒。[结果]芽外植体灭菌方式以5%NaClO_3 min+0.1%HgCl_2灭菌7 min+70%乙醇0.5 min组合效果最好;芽外植体消毒灭菌后在MS基本培养基中培养效果最好;6-BA、NAA对芽外植体的诱导和分化有促进作用,最佳诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.3 mg/L,此时芽分化成苗率最高。[结论]该研究可为费乌瑞它马铃薯种薯生产提供理论依据。

Study on Virus-free Technology of Favorita Potato

[Objective]To solve the problem of Favorita potato degradation.[Method]Detoxification of Favorita potato was studied by using plant stem tip virus-free culture technique.[Result]The combination of 5% NaClO 3 min +0.1%HgCl27 min +70%CH3CH2OH 0.5 min was suitable to sterile the buds of potato.After sterilizing the tuber and bud explants of potato,the best culture medium was MS.6-BA and NAA could promote the buds induction and differentiation.The optimal induction medium was MS +6-BA 0.5 mg/L +NAA 0.3 mg/L,at this point,the seedling rate was the highest.[ Conclusion] This study provides a theoretical basis for Favorita potato production.