4种抗生素对黑木耳黑29菌丝生长的影响

[目的]研究氨苄青霉素、硫酸链霉素、制霉菌素和卡那霉素对黑木耳外观特征及显微结构的影响,寻找适合的遗传筛选标记,同时又不降低黑木耳的产量与品质。[方法]以黑木耳黑29为试验材料,采用平板插片培养法、显微镜观察法测定菌丝生长速率、菌落形态特征和锁状联合菌丝数量。[结果]适宜浓度的青霉素和硫酸链霉素对黑29菌丝生长都有显著的促进作用,而制霉菌素和卡那霉素对菌丝生长有明显的抑制作用;制霉菌素和卡那霉素对菌丝色泽、密度影响较大,菌落隆起现象明显,偶尔出现褐变现象;随着4种抗生素浓度的升高,锁状联合菌丝比率先升高后降低,抗生素在50μg/mL时该比率升高显著。[结论]一定浓度的制霉菌素或卡那霉素较适合做遗传筛选标记,又不影响锁状联合菌丝的比率。该研究对黑木耳的遗传育种具有指导意义。

Effects of Four Kinds of Antibiotics on Mycelial Growth of Auricularia auricular H29

[Objective] To study the effect of ampicillin, streptomycin sulphate, nystatin and kanamycin on appearance characteristic and mi-crostructure in Auricularia auricular H29, to find a good genetic screening marker, without reducing the output and quality of the strain. [ Method] Mycelial growth rate, colonial morphological features and clamp connection hyphal amount were determined by plate culture and mi-croscopic method with Auricularia auricular H29 as the material.[ Result] A proper concentration of ampicillin and streptomycin sulfate signif-icantly promoted the mycelial growth, while nystatin and kanamycin significantly inhibited it.The nystatin and kanamycin had great effects on the color, lustre and density of mycelia.The colony uplift was obvious, and the browning phenomenon occasionally appeared.With the in-creasing of four antibiotics concentration, the ratio of hyphal clamp connection increased firstly and then decreased.It increased significantly at 50 μg/mL antibiotics concentration.[Conclusion] A certain concentration of nystatin or kanamycin is suitable for genetic screening markers while does not affect the ratio of hyphal clamp connection.This study is of guiding significance for the genetic breeding of black fungus.