大麻CBDA1基因的生物信息学分析

目的]研究大麻二酚酸合成酶基因(CBDA1)编码的蛋白质序列所包含的生物学信息。方法]利用生物信息学在线工具及软件分析大麻二酚酸合成酶(CBDAS)的理化性质、亲/疏水性、跨膜结构、信号肽、motif结构域及空间结构等。结果]CBDAS由544个氨基酸组成,分子量为62 168.42,理论等电点为8.81,是一种稳定的亲水性分泌蛋白,N末端包含1个由28个氨基酸残基组成的信号肽,该蛋白的亚细胞定位在胞外。CBDAS属于氧化还原酶家族,FDA是该酶活性的必需辅因子,CBDAS蛋白中只含有1个低复杂度区域,含有23个磷酸化位点和6个N-糖基化位点,其二级结构主要由α-螺旋、β-转角和无规卷曲组成,三级结构与四氢大麻酚酸合成酶(THCAS)同源性最高。结论]该研究结果可为今后深入研究CBDAS蛋白的结构特征和功能提供理论参考。

Bioinformatic Analysis of CBDA1 Gene in Cannabis sativa

Objective] To study the biological information contained in the protein sequence encoded by cannabidiolic acid synthase gene (CBDA1).Method]The physicochemical properties, hydrophilicity and hydrophobicity, transmembrane structure domain, the signal pep-tide, the motif and the spatial structure of cannabidiolic acid synthase (CBDAS) were analyzed by bioinformatic sever and online tools.Re-sult]CBDAS consisted of 544 amino acids with molecular weight of 62168.42 and a theoretical isoelectric point of 8.81.It was a stable hydro-philic secretory protein and the N-terminal contained a signal peptide of 28 amino acid residues, moreover, the subcellular localization of CB-DAS was extracellular.It was presumed that CBDAS belonged to the family of oxidoreductases and FDA was a necessary cofactor for the activi-ty of CBDAS.In addtion, CBDAS contained one low complexity region, twenty three phosphorylation sites and six N-glycosylation sites.The secondary structure mainly included α-helix,β-turn and random coil.Furthermore, the tertiary structure was the most homologous to tetrahy-drocannabinol synthase (THCAS).Conclution]The results could provide a theoretical reference for further study of the structure and func-tions of CBDAS.