亚硝化单胞菌is79a3 Sec途径分泌系统及底物蛋白分析

目的]分析亚硝化单胞菌is79a3 Sec途径分泌装置及底物蛋白。方法]从NCBI中选取亚硝化单胞菌is79a3菌株基因组注释的蛋白质氨基酸序列,采用Blast程序搜寻基因组中编码Sec途径分泌装置的蛋白,同时采用Signal P 4.0、Lipo P、TMHMM 2.0软件分析该菌株基因组中Sec途径的底物蛋白,同时采用COG功能数据库进行功能分析。结果]通过分析发现亚硝化单胞菌is79a3菌株编码Sec途径分泌装置有关的蛋白15个,包含Sec途径分泌系统中12个转运蛋白编码基因,1个信号肽识别蛋白编码基因和2个信号肽酶蛋白编码基因;Sec途径底物蛋白分析结果显示366个含有Sec途径Spase I类肽酶识别的信号肽,36个蛋白只含有Spase II类肽酶识别的信号肽。402个Sec途径底物蛋白功能分析结果显示:有272个蛋白功能不清楚,130个蛋白有具体的功能注释。结论]亚硝化单胞菌is79a3 Sec途径分泌装置完整,该菌株Sec途径底物蛋白大多数没有功能注释和功能未知,在有功能注释的蛋白中,主要参与细胞壁/细胞膜的生物合成、蛋白质翻译后修饰/蛋白更替、无机离子转运代谢、能源产生与转换。

Analysis of the Secretory System and Substrate Proteins of Sec Pathway of Nitrosomons is79a3

Objective] To study the secretory system and substrate proteins of sec pathway of Nitrosomons is79a3. Method] Genome se-quences of Nitrosomons is79a3 were used to predict to the secretory system of sec pathway by Blast software and then SignalP 4.0, LipoP, TM-HMM 2.0 softwares were used to analyze the substrate proteins of sec pathway.Function of substrate proteins was also analyzed by COG (Cluster of Orthologous Groups of proteins) database.Result] There were15 proteins of secretory system of sec pathway which included 12 transporters gene, 1 signal peptide recognition protein gene and two signal peptidase gene .Results of substrate proteins of sec pathway showed there were 366 proteins identified by type I Signal peptide enzyme ( Spase I ) and 36 proteins identified by type II signal peptide enzyme (Spase II).Function analysis of 402 proteins showed there were 272 proteins without specific function and 130 proteins had concrete function. Conclusion]The secretory system of sec pathway in genome of Nitrosomons is79a3 was complete and substrate proteins of sec pathway with an-notation of COG functions were mainly involved in cell wall/membrane biogenesis, posttranslational modification, protein turnover, inorganic ion transport and metabolism,energy production and conversion.