| 伪鹅观草高分子量麦谷蛋白基因启动子的克隆 |
| |
| 引用本文： | 李志新 曹双河 张相岐 张怀刚. 伪鹅观草高分子量麦谷蛋白基因启动子的克隆[J]. 长江大学学报, 2007, 4(2): 57-59,80 |
| |
| 作者姓名： | 李志新 曹双河 张相岐 张怀刚 |
| |
| 作者单位： | 长江大学农学院，中国科学院遗传与发育生物学研究所，中国科学院遗传与发育生物学研究所，中国科学院西北高原生物研究所 湖北荆州434025 中国科学院遗传与发育生物学研究所，北京100101，北京100101，北京100101，青海西宁810008 |
| |
| 基金项目： | 国家自然科学基因资助项目（30521001）和中国科学院资助项目（CXLY-2002-6） |
| |
| 摘 要： | 通过PCR克隆的方法,从二倍体伪鹅观草St基因组中克隆到了高分子量麦谷蛋白亚基基因Glu-1St1的启动子,该序列全长959bp,序列比对及结构分析表明,它与麦类高分子量麦谷蛋白亚基基因的启动子序列的一致性很高,达到82%,包含有典型麦谷蛋白亚基基因启动子的顺式作用元件。系统进化分析表明,它与大麦的D-hordein基因启动子的进化关系比较近,属于典型的x型亚基基因的启动子。
|
| 关 键 词： | 高分子量麦谷蛋白亚基基因 启动子 伪鹅观草(Pseudoroegneria stipifolia StSt) 克隆 |
| 文章编号： | 1673-1409（2007）02-S057-03 |
| 修稿时间： | 2007-05-08 |
Cloning the Promoter of High Molecular Weight Glutenin Subunit Gene in Pseudoroegneria stipifolia |
| |
| LI Zhi-xin College of Agronomy,Yangtze University,Jingzhou,Hubei ,China,State Key Laboratory of PlantCell and Chromosome Engineering. Cloning the Promoter of High Molecular Weight Glutenin Subunit Gene in Pseudoroegneria stipifolia[J]. Journal of Yangtze University, 2007, 4(2): 57-59,80 |
| |
| Authors: | LI Zhi-xin College of Agronomy Yangtze University Jingzhou Hubei China State Key Laboratory of PlantCell Chromosome Engineering |
| |
| Affiliation: | LI Zhi-xin College of Agronomy,Yangtze University,Jingzhou,Hubei 434025,China,State Key Laboratory of PlantCell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academyof Sciences,Beijing 100101,ChinaCAO Shuang-he,Zhang Xiang-qi State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Geneticsand Developmental Biology,Chinese Academy of Sciences,Beijing 100101,ChinaZHANG Huai-gang |
| |
| Abstract: | The promoter sequence with 959 bp of Glu-1St1 was obtained by genomic PCR experiment in diploid Pseudoroegneria stipifolia which contained St genome.The structural analysis indicated that the sequence had very high identity with the promoters of HMW-GS(high molecular weight glutenin subunits) genes in Triticum,and including an identical set of cis-elements found in promoter regions of previously characterized wheat HMW-GS genes.Phylogenetic analysis indicated that the promoter of Glu-1St1 was closely related with that of D-hordein,and clustered to x-type subunit gene promoters. |
| |
| Keywords: | HMW-GS gene promoter Pseudoroegneria stipifolia clone |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
