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Preparation of antigen for the counterimmunoelectrophoretic test for plasmacytosis in mink.
Authors:E Brummerstedt
Affiliation:Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Bulowsvej 13, DK-1870 Copenhagen, Denmark
Abstract:Counterimmunoelectrophoresis as a test method for making the diagnosis of plasmacytosis in mink demands the specific virus antigen. The method for preparation of the antigen according to Cho & Ingram (1972 a, b) with minor modifications is described in details, and results obtained at 62 antigen preparations are presented. In addition an ultrafiltration method is outlined which may be useful as a replacement for ultracentrifugation procedures used in the technique described by Cho & Ingram (1974).
Keywords:plasmacy tosis   mink   antigen   counterimmuno-electrophoresis
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