Cloning, expression and characterization of novel avenin-like genes in wheat and related species |
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Authors: | Peng Chen Changdong Wang Kexiu Li Junli Chang Yuesheng Wang Guangxiao Yang Peter R Shewry Guangyuan He |
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Institution: | aChina-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Key laboratory of Molecular Biophysics of Ministry of Education, Huazhong University of Science & Technology, Luoyu Road 1037, Wuhan 430074, China;bRothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK |
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Abstract: | B-Type avenin-like genes and proteins were characterized in 23 species of Triticeae. Southern blot analysis showed that the avenin-like genes belong to a multigene family. RT-PCR showed expression only in developing endosperms of wheat and related species, between 3 and 22 DPA (days post anthesis) with a peak between 11 and 15 DPA in wheat. The encoded proteins are cysteine-rich, containing 18–19 cysteine residues. An avenin-like protein from wheat was expressed in Escherichia coli, purified and used to raise polyclonal antibodies. These antibodies were used to detect b-type avenin-like proteins in endosperms of wheat and related species by western blotting. |
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Keywords: | Wheat Aegilops Avenin-like Seed storage protein |
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