| 黑龙江省犊牛腹泻粪便中轮状病毒的分离与鉴定 |
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| 引用本文： | 李昌仁,王君伟,李英霞,宣世伟,刘宝会,王红.黑龙江省犊牛腹泻粪便中轮状病毒的分离与鉴定[J].东北农业大学学报,1990(1). |
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| 作者姓名： | 李昌仁 王君伟 李英霞 宣世伟 刘宝会 王红 |
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| 作者单位： | 东北农学院兽医微生物教研室，东北农学院兽医微生物教研室，东北农学院兽医微生物教研室，东北农学院兽医微生物教研室，东北农学院兽医微生物教研室，东北农学院兽医微生物教研室 |
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| 摘 要： | 用MA—104细胞,以静止培养条件,从1月龄内的犊牛腹泻粪便中,分离出4电轮状病毒。经传至4～6代均使感染细胞出现特征性细胞病变。经直接电镜和免疫株镜观察,见有典型的轮状病毒颗粒,其RNA电泳型与NCDV株一致,以抗NCDV株高免血清琼扩检测及轮状病毒ELISA检测均为阳性。这4株病毒分别命名为DNRV_1,DNRV_2,DNRV_3,DNRV_4。
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| 关 键 词： | 黑龙江省 犊牛 腹泻粪便 轮状病毒 MA—104细胞 ELISA琼脂扩散试验 RNA电泳 |
ISOLATION AND IDENTIFICATION OF ROTAVIRUS FROM CALF'S FECES IN HEILONGJIANG PROVINCE |
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| Li Changren et al..ISOLATION AND IDENTIFICATION OF ROTAVIRUS FROM CALF''''S FECES IN HEILONGJIANG PROVINCE[J].Journal of Northeast Agricultural University,1990(1). |
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| Authors: | Li Changren |
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| Abstract: | Four rotavirus strains (designated DNRV-1, DNRV-2, DNRV-3, DNRV-4) were successfully isolated from the feces of about one-month-old calves with diarrhea. MA-104 cell and stationary-tube culture were used for the replication and isolation of the virus. After three or four generations in MA-104 cell, all the isolated strains could produce obvious cytopathic effects(CPE). The direct electron microscope and immune electron microscope revealed the presence of particles with typical rotavirus morphology; The isolated strains were identical with NCDV in RNA electrophoresis. By the methods of AGP and ELISA assay with anti-serum to NCDV, the results were positive in all the isolated strains. |
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| Keywords: | Heilongjiang province calf feces rotavirus MA-104 cell ELISA-AGP RNA electrophoresis |
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