利用SRY基因鉴定山羊胎儿成纤维细胞的性别

根据GenBank中已发表的山羊SRY基因和β-乳球蛋白基因序列设计2对PCR引物,对来自3个1月龄山羊胎儿成纤维细胞的基因组DNA进行PCR扩增,分别以公羊、母羊的基因组DNA为阳性和阴性对照,同时对PCR产物进行序列测定和同源性分析。结果表明,阳性对照和GFF1细胞系有2条扩增带,阴性对照、GFF2和GFF3细胞系只有1条扩增带;序列测定结果表明,PCR扩增产物为SRY基因,利用该方法准确鉴定出1个雄性细胞系和2个雌性细胞系。

Determination on Sex of Goat Fibroblast Cell with SRY Gene

According to the sequences of SRY gene and β-lactoglobulin gene of goat published in GenBank,2 pairs of PCR primers were designed,and the genome DNA from fibroblast cell of 3 one-month old goats were amplified by PCR.With genome DNA from male and female goats as the positive and negative CK resp.,the sequence of PCR product was determined and its homology was analyzed.The results showed that positive CK and GFF1 cell line had 2 amplified bands,negative CK,GFF2 and GFF3 cell lines only had 1 amplified band.The results of sequence determination showed that the PCR product was SRY gene,and with it 1 male cell line and 2 female cell lines were accurately identified.