| 番茄果实酸性转化酶基因cDNA片段的克隆 |
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| 引用本文： | 于喜艳,赵双宜,何启伟,侯丽霞.番茄果实酸性转化酶基因cDNA片段的克隆[J].中国蔬菜,2002,1(6):9-11. |
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| 作者姓名： | 于喜艳 赵双宜 何启伟 侯丽霞 |
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| 作者单位： | 山东农业大; 山东大学生命科学院; 山东农业科学院蔬菜研究所; |
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| 摘 要： | 根据酸性转化酶基因的保守序列设计引物 ,采用RT PCR方法 ,从番茄果实总RNA中扩增出目标cDNA片段 ,克隆到pMD18 T载体中。在所测的 10 38个核苷酸中 ,与GenBank中登记号为M810 81的番茄果实酸性转化酶基因高度同源 ,同源性为 99%。该基因片段在GenBank中已登记 ,登记号为AF4 90 5 30。
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| 关 键 词： | 番茄 RTPCR 酸性转化酶基因 |
| 收稿时间： | 2002-03-28; |
Cloning of a cDNA Fragment of Acid Invertase Gene from Tomato Fruit |
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| Yu Xiyan ,Zhao Shuangyi ,He Qiwei ,et al..Cloning of a cDNA Fragment of Acid Invertase Gene from Tomato Fruit[J].China Vegetables,2002,1(6):9-11. |
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| Authors: | Yu Xiyan Zhao Shuangyi He Qiwei |
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| Institution: | 1.College of Horticulture; Agriculture University; Tai′an 270118;2.College of Life Sciences; Shandong University;3.Vegetable Institute; Shandong Academy of Agricultural Sciences |
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| Abstract: | PCR primers were designed based on the consensus domain of some acid invertase genes in GenBank. A cDNA fragment was amplified from the total RNA isolated from toamto fruit via RT PCR and cloned into pMD18 T vector. The determined sequence showed that the amino acid sequence is 99% homologous to that of the corresponding region of acid invertase gene (Access No. M81081) in GenBank. This confirmed that we cloned a cDNA fragment from tomato fruit successfully and its access number is confirmed as AF490530 in GeneBank. |
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| Keywords: | Tomato fruit RT PCR Acid invertase gene |
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