盐胁迫对水稻萌芽中酶活性的影响

为了观测种子在萌芽过程中酶的动态变化规律,对水稻种子萌芽过程中在5个NaCl浓度(0、50、100、150、200 mmol/L)胁迫下的发芽率、淀粉酶、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和硝酸还原酶活性的变化进行测定.结果表明:在萌芽过程中随着盐胁迫程度加重,水稻种子发芽率、淀粉酶、SOD、CAT和硝酸还原酶活性均呈下降趋势.NaC1 150、200 mmlo/L处理的发芽率极显著低于空白对照,变幅在39％～100％;NaC1 100、200 mmol/L淀粉酶活性显著低于对照,NaC1 100 mmol/L处理的降幅在4.34％～49.13％,NaC1 200 mmol/L处理的变化幅度在-89.35％～119.73％;NaC1 100 mmol/L处理SOD活性显著下降,降幅在7.5％～50.19％,NaC1 50、100 mmol/L处理CAT活性降低显著,NaC1 150、200 mmol/L处理差异极显著,变幅在1.76％～50.61％之间;4个盐胁迫处理的硝酸还原酶活性下降不明显.

Effects of salt stress on germination of rice of enzyme activity

In order to observe dynamic changes of enzyme in the process of seed germination, the authors tested germination rate,amylase, superoxide dismutase(SOD), catalase(CAT) and nitrate reductase activity under 5 kinds of NaCl stress(0, 50, 100, 150, 200 mmol/L)in the rice seed germination. The results showed that in the process of seed germination, germination rate, amylase, SOD, CAT and nitratereductase activity decreased with the increasing of NaCl concentration. Under the NaCl concentration of 150 mmol/L and 200 mmol/L,germination rate was extremely significant lower than that of control group, amplituded by 39%~100%. Under the NaCl concentration of 100 mmol/L and 200 mmol/L, amylase was significant lower than that of the control group, decrease amplitude of 100 mmol/L and 200 mmol/L NaCl treatment were 4.34%~49.13% and -89.35%~119.73%, respectively. SOD activity decreased significantly in the 100 mmol/L NaCl treatment, amplituded by 7.5%~50.19%. CAT activity decreased significantly in 50 mmol/L and 100 mmol/L NaCl treatment, and were significantly different in 150 mmol/L and 200 mmol/L NaCl treatment, amplituded by 1.76%~50.61%. Nitrate reductase activity of 4 salt treatment decreased but not obvious.