Filamentous flexous particles and serologically related proteins of variable size associated with citrus psorosis and ringspot diseases |
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Authors: | Jesús Navas-Castillo Pedro Moreno |
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Institution: | (1) Instituto Valenciano de Inve stigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada, Valencia, Spain |
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Abstract: | Filamentous flexous partic les of unusual morphology, previously associated with several ringspot isolates, were detected also in psorosis A and psorosis B isolates by serologically specific electron microscopy using an antiserum to citrus ringspot. Upon partial purification of six ringspot, six psorosis A, and three psorosis B isolates, a specific protein of 47 kDa was detected in most cases, but two isolates (one psorosis A and one ringspot) had a 46 and a 48 kDa-protein, respectively. These differences in molecular masses were observed when purification was done from different host species or from plants co-inoculated with two isolates differing by their protein size. The three types of protein were serologically related in Western blots. Our results indicate that a common virus with different strains may be involved in psorosis A, psorosis B, and ringspot diseases. |
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Keywords: | serology electron microscopy electrophoresis mechanical transmission purification |
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