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葡萄二倍体和四倍体杂交胚挽救的影响因素
引用本文:郭印山,郭修武,张海娥,李轶晖,李成祥. 葡萄二倍体和四倍体杂交胚挽救的影响因素[J]. 果树学报, 2006, 23(1): 115-117
作者姓名:郭印山  郭修武  张海娥  李轶晖  李成祥
作者单位:沈阳农业大学园艺学院,沈阳,110161
基金项目:辽宁省科技攻关项目 , 辽宁省自然科学基金 , 沈阳农业大学校科研和教改项目
摘    要:采用L25(55)的正交试验设计,对葡萄二倍体和四倍体杂交胚挽救过程中的影响因素如培养基种类、接种时间、植物生长物质浓度等进行了研究。结果表明:影响胚挽救成苗率最重要的是接种时间,然后依次是IBA浓度、GA3浓度、培养基种类和6-BA浓度。获得了一批胚挽救实生苗,最高出苗率达到73.08%。

关 键 词:葡萄  二倍体和四倍体杂交  胚挽救  影响因素
文章编号:1009-9980(2006)01-115-03
收稿时间:2005-05-30
修稿时间:2005-09-09

Studies on the factors affecting embryo rescue of the crossed progeny between diploid and tetraploid grape cultivars
GUO Yin-shan,GUO Xiu-wu,ZHANG Hai-e,LI Yi-hui,LI Cheng-xiang. Studies on the factors affecting embryo rescue of the crossed progeny between diploid and tetraploid grape cultivars[J]. Journal of Fruit Science, 2006, 23(1): 115-117
Authors:GUO Yin-shan  GUO Xiu-wu  ZHANG Hai-e  LI Yi-hui  LI Cheng-xiang
Affiliation:College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161 China
Abstract:Using the straight cross experiment design of L25(55), the factors affecting the embryo rescue of crossed progeny between diploid and tetraploid grape cultivars embryo rescue, such as the kinds of culture medium, inoculated time, and plant growth substance concentration were studied. The result showed: the most important factor affecting the embryo rescue was inoculated time, then the concentration of IBA, GA3,the kinds of culture medium and the concentration of 6-BA.This experiment has achieved many seedlings through embryo rescue, and the highest rate of seedling received was 73.08%.
Keywords:Grape  Cross between diploid and tetraploid cultivars  Embryo rescue  Affecting factor
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