| AFLP在水稻类群划分研究中的应用 |
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| 引用本文： | 李本逊,蔡健,谭学林,徐绍中,周苏文.AFLP在水稻类群划分研究中的应用[J].种子,2001(4):26-27,53. |
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| 作者姓名： | 李本逊 蔡健 谭学林 徐绍中 周苏文 |
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| 作者单位： | 云南农业大学农学院,昆明,650201 |
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| 基金项目： | 云南省烟草公司资助项目 |
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| 摘 要： | 利用AFLP分子标记技术研究了22个水稻品种的遗传多样性，从49对AFLP引物中筛选出了4对多态性高，分辨能力强的引物，4个AFLP引物组合分别扩增出43、37、49、33条多态性带，平均每个引物组合扩出40．5条多态性带，4个引物组合共扩增出246条带。利用AFLP数据进行聚类分析，将22个品种聚为8群，结果表明，AFLP在水稻类群划分的应用是可行的。
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| 关 键 词： | AFLP 水稻品种 聚类分析 优势群 遗传多样性评价 作物育种 |
Application of AFLP Markers to Heterotic Grouping of Rice |
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| Li Benxun,Cai Jian,Tan Xuelin Xu Shaozhong,Zhou Suwen.Application of AFLP Markers to Heterotic Grouping of Rice[J].Seed,2001(4):26-27,53. |
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| Authors: | Li Benxun Cai Jian Tan Xuelin Xu Shaozhong Zhou Suwen |
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| Institution: | Faculty of Agricultural science and Technology of Yunnan Agricultural University 650201 |
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| Abstract: | AFLP marker technique waw used to study the diversity of 22 rice species. Four pairs of primers with high polymorphism and powerful distinctiveness were selected from 49 pairs of primer. Four AFLP primer combinations revealed 43,37,49 adn 33 AFLP polymorph bands, with an average of 40.5 bands produced per primer pair. Using 4 primer combinations could produce 246-AFLP bands. The cluster analysis based on AFLP dada showed that 22 rice species could be divided into 8 groups accorded with pedigree. The current Study indicated that it is practical to group rice species with AFLPs. |
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| Keywords: | Amplified fragment length polymorphism Rice species cluster analysis Heterotic group |
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