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Biological control of damping-off caused by Rhizoctonia solani using chitinase-producing Paenibacillus illinoisensis KJA-424 |
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| Authors: | WJ JungKN An YL JinRD Park KT LimKY Kim TH Kim |
| Institution: | a Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, South Korea b Department of Agricultural Chemistry, Biotechnology Research Institute and APSRC, College of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, South Korea c 521 Molecular Biochemistry Laboratory and Biodefensive Substances Group, Chonnam National University, Gwangju 500-757, South Korea |
| Abstract: | A bacterium having strong chitinolytic activity was isolated from a coastal soil in Korea and identified as Paenibacillus illinoisensis KJA-424 on the basis of the nucleotide sequence of a 16S rRNA gene. By activity staining after SDS-PAGE, three major chitinase bands with chitinolytic activity, approximate molecular weight of 63, 54 and 38 kDa were detected. On co-culture Rhizoctonia solani with KJA-424, abnormal swelling and deformation of R. solani hyphae were observed, where the release of N-acetyl-d-glucosamine was detected. The bacterium suppressed the symptom of damping-off cucumber seedlings caused by R. solani, in greenhouse trial. |
| Keywords: | Paenibacillus illinoisensis KJA-424 Chitinolytic activity Rhizoctonia solani Damping-off disease Cucumber seedlings |
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