| 激素因子对甘蔗愈伤组织诱导增殖与分化的效应 |
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| 引用本文： | 岑秀芬,杨燕妮,韦鹏霄,陈世民,彭芳,经东艳,严悦.激素因子对甘蔗愈伤组织诱导增殖与分化的效应[J].广西农业科学,2010,41(9):889-892. |
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| 作者姓名： | 岑秀芬 杨燕妮 韦鹏霄 陈世民 彭芳 经东艳 严悦 |
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| 作者单位： | 广西大学农学院,南宁,530005 |
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| 摘 要： | 对巴西固氮甘蔗品种B8（RB86-7515）、新台糖22号（ROC22）嫩叶鞘进行离体培养,以MS为基本培养基,研究不同激素种类及组合对愈伤组织诱导、增殖和分化的效应。结果表明,以2,4-D3.0mg/L＋NAA0.2mg/L＋KT1.0mg/L激素组合的愈伤组织诱导率最高,ROC22和B8的诱导率分别达92.67%、88.89%;以2,4-D3.0mg/L＋NAA0.2mg/L＋KT0.5mg/L激素组合的愈伤组织增殖效果最好,ROC22和B8的愈伤组织增殖倍数分别为3.5和2.5倍;以NAA0.1mg/L＋6-BA1.5mg/L＋TDZ0.04mg/L激素组合的甘蔗愈伤组织分化效果最好,ROC22和B8的芽分化率分别为82.22%和77.78%。
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| 关 键 词： | 甘蔗 离体培养 激素因子 愈伤组织 |
Effect of hormonal factors on callus induction, proliferation and differentiation in sugarcane |
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| CEN Xiu-fen,YANG Yan-ni,WEI Peng-xiao,CHEN Shi-min,PENG Fang,JING Dong-yan,YAN Yue.Effect of hormonal factors on callus induction, proliferation and differentiation in sugarcane[J].Guangxi Agricultural Sciences,2010,41(9):889-892. |
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| Authors: | CEN Xiu-fen YANG Yan-ni WEI Peng-xiao CHEN Shi-min PENG Fang JING Dong-yan YAN Yue |
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| Institution: | CEN Xiu-fen,YANG Yan-ni,WEI Peng-xiao,CHEN Shi-min,PENG Fang,JING Dong-yan,YAN Yue(Agricultural College,Guangxi University,Nanning 530005,China) |
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| Abstract: | The young leaf sheaths of two sugarcane varieties B8(RB86-7515) and ROC22 were cultured in vitro to study the effects of different hormone combinations on the callus induction,proliferation and differentiation.The results indicated that the callus induction rate of explants was higher in ROC22(92.67%) compared to the variety B8(88.89%) when cultured in MS medium supplied with 2,4-D(3.0 mg/L),NAA(0.2 mg/L) and KT(1.0 mg/L).The proliferation times of callus in two sugarcane varieties reached the highest for v... |
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| Keywords: | sugarcane in vitro culture hormone factor callus |
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