高浓度6-BA诱导酸樱桃苗的玻璃化苗内源激素含量变化

研究了培养基中不同浓度6-BA对酸樱桃组培苗玻璃化率及内源激素含量的影响,利用酶联免疫吸附分析法测定正常苗与玻璃化苗愈伤组织和芽中IAA、GA3和ZR含量.结果表明:高浓度6-BA导致组培苗玻璃化,当培养基中6-BA浓度为0.5 mg·L-1时,组培苗未出现玻璃化苗;当培养基中6-BA浓度为3.0 mg·L-1时,组培苗玻璃化率高达95%.通过玻璃化苗和正常苗内源激素比较发现,玻璃化苗愈伤组织IAA和GA3含量明显高于正常苗愈伤组织中IAA和GA3含量,ZR含量基本相等;玻璃化苗芽中ZR含量远远低于正常苗中ZR含量,而IAA和GA3含量基本不变,高浓度6-BA导致组培苗内源激素比例失调发生玻璃化.

Effect of High Level of 6-BA on the Endogenous Hormones Level Changes in the Vitrification Seedling of Prunus cerasus

This paper studied the effects of different levels of 6-BA on the vitrification rate of Prunus cerasus and the changes of endogenous hormones level,determined endogenous hormones content in the buds and callus by EnZRyme-linked Immunosorbent Assays.The results showed that the high level of 6-BA would raise the vitrification rate;as 6-BA content was 0.5 mg·L^-1,the vitrification rate was 0,as 6-BA content added to 3.0 mg·L^-1,the vitrification rate was 95%.In callus of the vitrification seedling,IAA and GA₃level were higher than that of the normal seedling,ZR content was the same;In buds of the vitrification seedling,ZR level was lower than that of the normal seedling,IAA and GA₃ contents were equal.The high level of 6-BA would cause the dislocation of the endogenous hormones rate in the vitrification seedling.