| 基于近等基因导入系发掘玉米抗甘蔗花叶病毒主效基因 |
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| 引用本文： | 吕香玲, 李新海, 郝转芳, 吉海莲, 史利玉, 张世煌. 基于近等基因导入系发掘玉米抗甘蔗花叶病毒主效基因[J]. 玉米科学, 2007, 15(3): 009-014 |
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| 作者姓名： | 吕香玲 李新海 郝转芳 吉海莲 史利玉 张世煌 |
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| 作者单位： | 1. 沈阳农业大学农学院,沈阳,110161;中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程/农作物遗传育种重点开放实验室,北京,100081
2. 中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程/农作物遗传育种重点开放实验室,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划);引进国际先进农业科学技术计划项目 |
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| 摘 要： | 利用玉米自交系掖478与中自01构建近等基因导入系群体(BC4F2),通过田间人工接种甘蔗花叶病毒鉴定获得抗病植株。采用38个SSR标记分析抗病株基因型,通过连锁不平衡分析,在玉米第3和6染色体上发掘3个主效抗病QTL。第3染色体上的QTL置信区间为26.1cM-phi053-5cM;第6染色体上的QTL置信区间分别为1.2cM-bnlg161和5.3cM-bnlg1538-7cM。建立了基于近等基因导入系发掘玉米抗甘蔗花叶病毒主效QTL技术,获得了一批含有抗病毒QTL的近等基因导入系,为抗病育种提供了信息和材料。
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| 关 键 词： | 玉米 近等基因导入植株 甘蔗花叶病毒 抗病基因 |
| 文章编号： | 1005-0906（2007）03-0009-06 |
| 收稿时间： | 2006-12-11 |
| 修稿时间： | 2006-12-11 |
Identification of Major QTL for Resistance to Sugarcane Mosaic Virus in Maize Based on Introgression Lines Analysis |
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| LU Xiang-ling, LI Xin-hai, HAO Zhuan-fang, ZHANG Shi-huang,et al.. Identification of Major QTL for Resistance to Sugarcane Mosaic Virus in Maize Based on Introgression Lines Analysis[J]. Journal of Maize Sciences, 2007, 15(3): 009-014 |
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| Authors: | LU Xiang-ling LI Xin-hai HAO Zhuan-fang ZHANG Shi-huang et al. |
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| Affiliation: | 1. College of A gronomy, Shengyang Agricultural University, Shengyang 110161; 2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081, China |
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| Abstract: | In this paper,36 resistant introgression plants were selected from a BC4F2 population of 270 plants derived from a cross between Ye478(susceptible, recurrent parent) and Zhongzi01(resistant, donor parent) by artificially inoculating sugarcane mosaic virus(SCMV) in the field, and genotyped by 38 SSR markers. Using linkage disequilibrium mapping method, three major QTL for SCMV resistance were identified, one on chromosome 3 and two on chromosome 6. The confidence interval of QTL on chromosome 3 was located on 26.1cM-phi053-5cM, and that on chromosome 6 were on 1.2 cM-bnlg161 and 5.3cM-bnlg1538-7cM, respectively. The procedure for identifying major QTL for SCMV resistance was established based on BC4F2 introgression plants or lines analysis by SSR markers, and a number of plants/lines with SCMV resistant QTL were obtained, which provided both useful genetic information and materials in marker assisted selection for SCMV resistance in maize. |
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| Keywords: | Maize Introgression plant Sugarcane mosaic virus Disease resistance gene |
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