霍霍巴组培快繁技术体系研究 Ⅱ.分化和生根培养中的激素配比

研究了霍霍巴茎段初代培养、继代培养和生根培养 3个技术环节的激素配比。结果表明 :以改良MS为基本培养基 ,初代培养的激素配比以 6-BA 2mg/L +GA30 .5mg/L +IBA0 .0 5mg/L为宜 ,诱芽率 1 0 0 %,增殖系数 4.9;在继代培养中 ,若用单激素 6-BA ,1mg/L时即可维持较高的增殖系数 ( 7.8) ;如用 2mg/L 6-BA和 0 .5mg/LGA3,增殖系数达 8.3,可见低浓度GA3和 6-BA组合可以促进霍霍巴多芽的分化 ;使用 6-BA的浓度不宜超过 3mg/L ,因为较高的细胞分裂素明显增加了组培苗超度含水态的比率 ;以改良 1 / 2MS为基本培养基 ,生根培养的激素配比为 3mg/LNAA + 1 .5mg/LIBA + 0 .5mg/LIAA ,培养 2 0d时的生根率达 83%。

Studies on the Technique System of Tissue Culture and Rapid Propagation of Jojoba Ⅱ.Various Hormone Combinations in the Differentiation and Rooting Culture

Studies on hormone combinations of 3 kinds of cultures including primary culture, subculture and rooting culture in the micropropagation method of jojoba showed that the axillary buds of nodal segments were induced to proliferation on modified MS medium supplemented with 2 mg/L 6-BA, 0.5 mg/L GA_3 and 0.05 mg/L IBA.On an average, 4.9 shoots were formed per original explant in the initial cultures.The number of shoots in subsequent culture reached 7.8 on 1 mg/L 6-BA-containing medium. If using the combination of 2 mg/L6-BA and 0.5 mg/L GA_3,the multiplication coefficient reached 8.3. It indicated that 6-BA combining with low concentration of GA_3 promoted the shoots propagation.6-BA shouldn't exceed 3 mg/L, because high concentration of cytokinin enhanced the ratio of hyper hydricity.After culture for 20 days on the 1/2 modified MS medium supplemented with 3 mg/L IBA, 1.5 mg/L NAA and 0.5 mg/L IAA, about 83% of the shoots had developed roots.