| 摘 要： | Exploitation of the high yield potential intersubspecific heterosis has so far been hampered due to primarily the existence of high femalesterility. We have offered a cytological explanation for this abortion, such as the absence of embryo sacs or the occurrence of empty embryo sacs. Here we report the ultrastructural changes and ultracytochemical localization of acid phosphatase(AcPase) in the abortive functional megaspores. The F_1 hybrid was derived from a cross, Liaoqiu 5 (japonica)×Zhenjinzhen (indica), which showed high seed sterility in F_1 plants. The tissue prepared for enzyme ultracytochemicl study was fixed in 2.5% glutaraldehyde and 4% paraformaldehyde, incubated at 20℃ in the complete reaction medium of Barka-Anderson for 2h, post-fixed in 1% OsO_4 before ethanol-dehydration, infiltration and embedding in Epon 812. The sections, cut on. LKB-2088V ultramicrotome, were viewed and photographed, either unstained (in the case of AcPase localization) or double-stained with urayl acetate and lea
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