Cryptosporidium meleagridis in an Indian ring-necked parrot (Psittacula krameri) |
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Authors: | Morgan U M Xiao L Limor J Gelis S Raidal S R Fayer R Lal A Elliot A Thompson R C |
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Institution: | World Health Organisation Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Western Australia. |
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Abstract: | OBJECTIVE: To perform a morphological and genetic characterisation of a Cryptosporidium infection in an Indian ring-necked parrot (Psittacula krameri) and to compare this with C meleagridis from a turkey. DESIGN: Tissue and intestinal sections from an Indian ring-necked parrot were examined microscopically for Cryptosporidium. The organism was also purified from the crop and intestine, the DNA extracted and a portion of the 18S rDNA gene amplified, sequenced and compared with sequence and biological information obtained for C meleagridis from a turkey as well as sequence information for other species of Cryptosporidium. RESULTS: Morphological examination of tissue sections from an Indian ring-necked parrot revealed large numbers of Cryptosporidium oocysts attached to the apical border of enterocytes lining the intestinal tract. Purified Cryptosporidium oocysts measured about 5.1 x 4.5 microns, which conformed morphologically to C meleagridis. The sequence obtained from this isolate was identical to sequence information obtained from a C meleagridis isolate from a turkey. CONCLUSION: Cryptosporidium meleagridis was detected in an Indian ring-necked parrot using morphological and molecular methods. This is the first time that this species of Cryptosporidium has been reported in a non-galliform host and extends the known host range of C meleagridis. |
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Keywords: | Cryptosporidium meleagridis Indian ring-necked parrot turkey clinical history microscopy 18S rDNA gene |
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