| 一种简单高效提取棉花不同组织总RNA的方法 |
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| 引用本文： | 李晶,王亦学,郑德刚,梁爱华,罗晓丽,吴家和. 一种简单高效提取棉花不同组织总RNA的方法[J]. 山西农业科学, 2009, 37(5): 17-19 |
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| 作者姓名： | 李晶 王亦学 郑德刚 梁爱华 罗晓丽 吴家和 |
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| 作者单位： | 山西大学生物技术研究所,山西,太原,030006;中国科学院微生物研究所植物基因组学国家重点实验室,北京,100101;山西省农业科学院棉花研究所,山西,运城,044000;山西大学生物技术研究所,山西,太原,030006;中国科学院微生物研究所植物基因组学国家重点实验室,北京,100101 |
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| 摘 要： | 目前,虽然已经报道了几种比较有效的提取棉花组织总RNA的方法,但是这些方法往往费时费工,又很难同时适用于棉花不同组织的总RNA提取。以CTAB法为基础进行技术改良,同时结合LiCl沉淀等,总结出了一种简单、快速又普遍适用于棉花各组织总RNA的提取方法。试验证明,此法提取到的RNA纯度高、完整性好,适用于逆转录合成cDNA第一链、RT-PCR分析以及Northern杂交等分子实验。
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| 关 键 词： | 棉花 RNA 提取 |
A Simply and High Efficiency Method of Total RNA Isolation in Upland Cotton |
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| LI Jing,WANG Yi-xue,ZHENG De-gang,LIANG Ai-hua,LUO Xiao-Li,WU Jia-he. A Simply and High Efficiency Method of Total RNA Isolation in Upland Cotton[J]. Journal of Shanxi Agricultural Sciences, 2009, 37(5): 17-19 |
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| Authors: | LI Jing WANG Yi-xue ZHENG De-gang LIANG Ai-hua LUO Xiao-Li WU Jia-he |
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| Affiliation: | LI Jing, WANG Yi-xue, ZHENG De-gang, LIANG Ai-hua, LUO Xiao-Li,WU Jia-he ( 1.Institute of Biotechnology,Shanxi University, Taiyuan 030006,China; 2.State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences ,Beijing 100101, China; 3.Cotton Research Institute ,Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China) |
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| Abstract: | At present several methods of cotton RNA isolation were reported, but they were time and labor consuming, and incompatible for all cotton tissues. Based on the CTAB extraction method and the LiCI precipitation method for total RNA, a fast and simply method for cotton total RNA isolation was developed, which is also suitable for most of cotton tissues. The cotton total RNA produced by this method was high purity, integrality, and could be used in reverse transcription expression analysis, RT-PCR analysis and Northern blotting etc. |
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| Keywords: | RNA |
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