独蒜兰共生菌根真菌动态变化研究

采用兰科植物菌根真菌单菌丝团分离方法,对独蒜兰盛花期和盛叶期菌根真菌进行了分离培养,结果发现根形成早期、根伸长区和近根尖成熟区、外皮层细胞中以生长快、气生菌丝发达的印Epulorhiza属菌根真菌为主,成熟根、近根基部成熟区、皮层中部及内皮层细胞中以生长慢、气生菌丝不发达的Epulorhiza属菌根真菌为主.生长快、气生菌丝发达的Epulorhiza属菌根真菌对寄主的侵染力强,对菌根的形成及独蒜兰的生长发育起着关键性的作用;生长慢、气生菌丝不发达的菌根真菌对寄主的侵染力弱,对菌根的维持起重要作用;这两类Epulorhiza属菌根真菌协同作用,共同促进菌根的形成和维持,这为组培苗的菌根化提供了一个新的思路.

Study on Dynamic Changes of Symbiotic Mycorrhizal Fungi in Roots of Pleione bulbocodioides

Symbiotic Rhizoctonia in roots of Pleione bulbocodioides from flowering and leaving stages were isolated from a single peloton. Fast growing Epulorhiza sp. with aerial hyphae (FGEAH) were the predominant fungi in tender roots and slow growing Epulorhiza spp. without aerial hyphae(SGEAH) were the predominant fungi in mature roots/The predominant fungi in cells of exodermis,elongation zone and maturation zone near root tip were FGEAHs and those in cells of middle cortex,maturation zone near root rhizel were SGEAHs.The FGEAHs played a key role in mycorrhizal formation,growth and development of P. bulbocodioide. The SGEAHs were important for keeping the mycorrhizal symbiosis. The formation and maintenance of mycorrhizal symbiosis were promoted by cooperation between SGEAHs and FGEAHs. A new idea was given for mycor-rhizalization of tissue culture seedlings.