N-月桂酰乙醇胺对香石竹开放和衰老进程中花瓣微粒体膜组分和功能的调节

 【目的】探讨N-月桂酰乙醇胺N-lauroylethanolamine, NAE（12:0）]延缓香石竹切花衰老的作用机理。【方法】以5 μmol?L-1 NAE（12﹕0）对香石竹切花（Dianthus caryophyllus L.）‘Red Barbara’进行瓶插处理，研究瓶插试验进程中NAE（12﹕0）对花瓣微粒体膜组分和功能的影响。【结果】香石竹切花开放和衰老进程伴随着花瓣微粒体膜磷脂含量、膜脂流动性、膜脂脂肪酸不饱和指数以及膜结合酶比活力的下降，外源NAE（12﹕0）处理能在切花盛开后期至初萎发生前明显滞缓香石竹花瓣微粒体膜上述指标的下降速率，同时降低作为膜衰老可靠指标的电导率的上升幅度。【结论】NAE（12:0）延缓香石竹切花衰老的作用机理与NAE（12﹕0）对花瓣微粒体膜磷脂降解以及膜脂脂肪酸组分的调节有关，由此延缓了花瓣微粒体膜脂流动性的下降速率，降低了膜渗漏的上升幅度，并维持了膜结合酶较高的比活力，从而推迟了切花初萎的发生。

Regulatory Role of N-lauroylethanolamine on Microsomal Membrane Composition and Function in Petals of Carnation During the Blossoming and Senescence

The present study was conducted to examine the possible involvement of N- lauroylethanolamine(NAE(12:0)) in the regulation of petal-membrane senescence. The petal-membrane senescence is accompanied by a decrease in membrane phospholipids content, membrane fluidity, the index of unsaturation of fatty acids and activities of membrane enzymes, and NAE(12:0) supplementation delayed the decreases in above physiology parameters in the aging petals in Dianthus caryophyllus L. ‘Red Barbara’. Electrolyte leakage, which is a reliable indicator of petal-membrane senescence, was also postponed in NAE(12:0)-treated flowers. The data collectively suggest that the NAE(12:0)-induced delay in petal-membrane senescence involved the regulation on the degradation of phospholipids and changes of the fatty acid composition of microsomal membranes, which resulted in the less ion leakage, more fluidity of membrane and higher activities of membrane enzymes, thus preserving the integrity of the petal-membrane and delaying the initial wilting of cut carnation.