| 宁夏枣树品种品系的AFLP分析 |
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| 引用本文： | 魏天军,李彦龙,李百云.宁夏枣树品种品系的AFLP分析[J].中国农学通报,2010,26(10):239-241. |
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| 作者姓名： | 魏天军 李彦龙 李百云 |
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| 作者单位： | 1. 宁夏农林科学院种质资源研究所,银川,750002
2. 宁夏枸杞工程技术研究中心,银川,750002 |
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| 基金项目： | 宁夏5183农业工程重大专项“枣树品种选育及无病毒苗木培育技术研究”中的部分内容。 |
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| 摘 要： | 摘 要： 应用基因组DNA-AFLP分子标记技术，对原产宁夏回族自治区的8个枣树品种品系和原产甘肃靖远县的1个枣树品种的亲缘关系进行了研究。研究结果表明，5对引物（E1/M12、E1/M4、E4/M11、E2/M10和E5/M4）扩增出了466条带，其中多态性条带为130条，多态性比率达27.89%，表明遗传多样性不是很丰富。聚类结果证明灵武长枣2号与中卫大枣、同心圆枣聚为一类，彼此间近缘关系较近，而与灵武长枣品种的亲缘关系较远，是一个新种质；同心圆枣与中卫大枣的遗传距离仅为0.015，亲缘关系最近，是同物异名。
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| 关 键 词： | 胸腺素α1 胸腺素α1 大肠杆菌 可溶性表达 |
| 收稿时间： | 2009-12-17 |
| 修稿时间： | 2010-01-06 |
AFLP analysis on jujube cultivars and strains in Ningxia Autonomous Region |
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| Wei Tianjun,Li Yanlong,Li Baiyun.AFLP analysis on jujube cultivars and strains in Ningxia Autonomous Region[J].Chinese Agricultural Science Bulletin,2010,26(10):239-241. |
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| Authors: | Wei Tianjun Li Yanlong Li Baiyun |
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| Institution: | 1Institute of Germplasm Resource, Yinchuan 750002; 2Engineering and Technology Research Center of Ningxia Wolfberry,
Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002 |
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| Abstract: | The genetic relationships among 9 jujube cultivars and strains were analyzed using the technique of genomic DNA-AFLP markers in Ningxia Hui People’s Autonomous Region. Five primer pairs （E1/M12, E1/ M4, E4/M11, E2/M10 and E5/M4） were used for the analysis. A total of 466 AFLP markers were identified, of which 130 were polymorphic, and polymorphism percentage was 27.89%. Using UPGMA （un-weighted pair group method arithmetic average） clustering method, 9 cultivars and strains were placed into 2 clusters. ‘Lingwuchangzao No.2’selected from lingwuchangzao jujubes had farther genetic distance, that is a new germplasm of jujube.‘Tongxinyuanzao’jujube and‘zhongweidazao’jujube were very close genetically, being the same cultivar. |
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| Keywords: | Ningxia Autonomous Regionzz |
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