猪繁殖与呼吸综合征抗病育种研究进展

猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome, PRRS)是由猪繁殖与呼吸综合征病毒(PRRS virus, PRRSV)引起的一种高度接触性传染病,严重危害我国乃至世界养猪业。然而由于PRRSV抗原的多变性,目前包括疫苗接种、药物治疗等在内的防治措施效果不佳。因此,随着现代分子生物学技术的不断发展,基于基因编辑技术对猪PRRS的抗病育种逐渐发展起来。本文简述了PRRS的临床症状,重点回顾了国内外PRRS抗病育种研究进展,通过分析PRRS的致病机制,重点阐述了PRRSV受体及针对不同受体进行编辑的体内及体外抗病毒效果,以期为未来深入研究PRRSV致病机制、开发PRRS抗病品种提供理论依据。

Advances of Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRS virus, PRRSV), which seriously endangers the pig industry in China and even the world. However, due to the variability of PRRSV antigen, the current prevention and control measures, including vaccination and drug treatment, are ineffective. Therefore, with the continuous development of modern molecular biology technology, the disease-resistant breeding of PRRS in pigs based on gene editing technology has gradually developed. This paper briefly describes the clinical symptoms of PRRS, and focuses on the research progress of PRRS disease-resistance breeding at home and abroad. By analyzing the pathogenic mechanism of PRRSV, the PRRSV receptors and the in vivo and in vitro antiviral effects of editing against different receptors were emphasized to provide a theoretical basis for further research of the pathogenic mechanism of PRRSV and the development of PRRS-resistant varieties in the future.