苦参苍术颗粒对大肠杆菌感染肉鸡肝组织胆固醇代谢的调节作用

旨在探究苦参苍术颗粒是否通过调节肝组织胆固醇代谢,改善致病性大肠杆菌诱导的肉鸡肝组织胆固醇合成异常。将40只21日龄白羽肉鸡随机分为空白对照组、模型对照组、苦参苍术低剂量组、中剂量组和高剂量组,每组8只。模型对照组和苦参苍术组分别胸部肌肉注射0.7 mL致病性大肠杆菌菌液(8.37×10⁸ CFU·mL^-1),苦参苍术组在接种菌液24 h后,在饮水中分别添加不同剂量的苦参苍术颗粒(低剂量组：3.6 g·L^-1,中剂量组：5.5 g·L^-1,高剂量组：7.3 g·L^-1),自由饮水,连用7 d。试验结束,所有试验肉鸡处死,收集血液,采集组织样品,称体重和各器官重;采用石蜡切片技术观察肝组织形态变化;检测血清和肝组织中总胆固醇(total cholesterol, TCH)、甘油三酯(hepatic triglyceride, TG)、高密度脂蛋白胆固醇(high-density lipoprotein-cholesterol, HDL-C)、低密度脂蛋白胆固醇(low-densi...

Regulation of Kushen-cangzhu Granules on Hepatic Cholesterol Metabolism in Broilers Infected with Escherichia coli

The aim of this experiment was to investigate whether kushen-cangzhu (Sophora flavescens Ait. and Atractylodes Lancea) granules could improve the abnormal cholesterol synthesis induced by Escherichia coli (E. coli) through regulating liver cholesterol metabolism in broilers. Forty 21 days old white-feathered broilers were randomly divided into 5 groups (8 in each group): Blank control group, model control group, low-, medium-and high-dose of kushen-cangzhu groups. The model control group and different doses of kushen-cangzhu groups were intramuscular thoracic injection treated with pathogenic E. coli bacterial solution for 0.7 mL (8.37×10⁸ CFU·mL^-1). After inoculating pathogenic E. coli bacterial solution for 24 h, different doses of kushen-cangzhu granules (low-dose group: 3.6 g·L^-1, medium-dose group: 5.5 g·L^-1, high-dose group: 7.3 g·L^-1) were supplemented in drinking water for chickens in low-, medium-and high-dose groups, respectively, and the trail lasted for 7 days. At the end of the experiment, all the broilers were slaughtered, body and organs were weighed, blood and tissue samples were collected. The morphological changes of liver were observed by hematoxylin-eosin (HE) staining. The levels of serum and liver total cholesterol (TCH), hepatic triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were detected, RT-qPCR was used to detect the expression of cholesterol metabolism-related genes in liver. The results showed that, compared with the blank control group, the broilers in the model control group had significantly lower body weight, higher liver index and serum TG content (P＜0.05), while infiltrated inflammatory cells in the central vein and the hepatic sinusoid and disordered hepatocyte was observed in model control group. TG and SCAP mRNA expression was significantly lower (P＜0.05) in liver of model control group compared with blank control group. Compared with the model control group, the body weight and liver LDL-C content was significantly increased in the low-dose group (P＜0.05), while the liver index and serum TG content were significantly decreased (P＜0.05), and the hepatic morphological structure was at a better state. The HDL-C, LDL-C and the LDL-C/HDL-C ratio in liver were significantly increased in the medium-dose group compared with model control group. Meanwhile, the mRNA expression of HMGCR, SREBP2 and SCAP was significantly enhanced (P＜0.05) in medium-dose group than model control group. In the high-dose group, the serum TG concentration was significantly reduced (P＜0.05) but hepatic LDL-C was significantly increased (P＜0.05), and the ratio of LDL-C/HDL-C was significantly raised (P＜0.05) compared with the model control group. In conclusion, the liver abnormal synthesis of cholesterol in broilers induced by E. coli was alleviated by kushen-cangzhu granules through regulating cholesterol metabolic pathway.